3AUF: Crystal Structure Of Glycinamide Ribonucleotide Transformylase 1 From Symbiobacterium Toebii

The crystal structures of glycinamide ribonucleotide transformylases (PurNs) from Aquifex aeolicus (Aa), Geobacillus kaustophilus (Gk) and Symbiobacterium toebii (St), and of formyltetrahydrofolate hydrolase (PurU) from Thermus thermophilus (Tt) were determined. The monomer structures of the determined PurN and PurU were very similar to the known structure of PurN, but oligomeric states were different; AaPurN and StPurN formed dimers, GkPurN formed monomer and PurU formed tetramer in the crystals. PurU had a regulatory ACT domain in its N-terminal side. So far several structures of PurUs have been determined, yet, the mechanisms of the catalysis and the regulation of PurU have not been elucidated. We, therefore, modelled ligand-bound structures of PurN and PurU, and performed molecular dynamics simulations to elucidate the reaction mechanisms. The evolutionary relationship of the two enzymes is discussed based on the comparisons of the structures and the catalytic mechanisms.
PDB ID: 3AUFDownload
MMDB ID: 97734
PDB Deposition Date: 2011/2/3
Updated in MMDB: 2012/03
Experimental Method:
x-ray diffraction
Resolution: 2.07  Å
Source Organism:
Similar Structures:
Biological Unit for 3AUF: dimeric; determined by author and by software (PISA)
Molecular Components in 3AUF
Label Count Molecule
Proteins (2 molecules)
Glycinamide Ribonucleotide Transformylase 1
Molecule annotation
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