3AL0: Crystal Structure Of The Glutamine Transamidosome From Thermotoga Maritima In The Glutamylation State

Citation:
Abstract
In most bacteria and all archaea, glutamyl-tRNA synthetase (GluRS) glutamylates both tRNA(Glu) and tRNA(Gln), and then Glu-tRNA(Gln) is selectively converted to Gln-tRNA(Gln) by a tRNA-dependent amidotransferase. The mechanisms by which the two enzymes recognize their substrate tRNA(s), and how they cooperate with each other in Gln-tRNA(Gln) synthesis, remain to be determined. Here we report the formation of the 'glutamine transamidosome' from the bacterium Thermotoga maritima, consisting of tRNA(Gln), GluRS and the heterotrimeric amidotransferase GatCAB, and its crystal structure at 3.35 A resolution. The anticodon-binding body of GluRS recognizes the common features of tRNA(Gln) and tRNA(Glu), whereas the tail body of GatCAB recognizes the outer corner of the L-shaped tRNA(Gln) in a tRNA(Gln)-specific manner. GluRS is in the productive form, as its catalytic body binds to the amino-acid-acceptor arm of tRNA(Gln). In contrast, GatCAB is in the non-productive form: the catalytic body of GatCAB contacts that of GluRS and is located near the acceptor stem of tRNA(Gln), in an appropriate site to wait for the completion of Glu-tRNA(Gln) formation by GluRS. We identified the hinges between the catalytic and anticodon-binding bodies of GluRS and between the catalytic and tail bodies of GatCAB, which allow both GluRS and GatCAB to adopt the productive and non-productive forms. The catalytic bodies of the two enzymes compete for the acceptor arm of tRNA(Gln) and therefore cannot assume their productive forms simultaneously. The transition from the present glutamylation state, with the productive GluRS and the non-productive GatCAB, to the putative amidation state, with the non-productive GluRS and the productive GatCAB, requires an intermediate state with the two enzymes in their non-productive forms, for steric reasons. The proposed mechanism explains how the transamidosome efficiently performs the two consecutive steps of Gln-tRNA(Gln) formation, with a low risk of releasing the unstable intermediate Glu-tRNA(Gln).
PDB ID: 3AL0Download
MMDB ID: 85169
PDB Deposition Date: 2010/7/19
Updated in MMDB: 2010/10
Experimental Method:
x-ray diffraction
Resolution: 3.37  Å
Source Organism:
Thermotoga maritima MSB8
Similar Structures:
Biological Unit for 3AL0: tetrameric; determined by software (PISA)
Molecular Components in 3AL0
Label Count Molecule
Proteins (3 molecules)
1
Glutamyl-trna(gln) Amidotransferase Subunit a(Gene symbol: gatA)
Molecule annotation
1
Aspartyl/glutamyl-trna(asn/gln) Amidotransferase Subunit B(Gene symbol: gatB)
Molecule annotation
1
Glutamyl-trna(gln) Amidotransferase Subunit C,linker, Glutamate--trna Ligase 2
Molecule annotation
Nucleotide(1 molecule)
1
Trnagln
Molecule annotation
Chemicals (2 molecules)
1
1
2
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.