3A2X: Peroxiredoxin (c50s) From Aeropyrum Pernix K1 (acetate-bound Form)

Citation:
Abstract
Peroxiredoxin (Prx) reduces hydrogen peroxide and alkyl peroxides to water and corresponding alcohols, respectively. The reaction is dependent on a peroxidatic cysteine, whose sulphur atom nucleophilically attacks one of the oxygen atoms of the peroxide substrate. In spite of the many structural studies that have been carried out on this reaction, the tertiary structure of the hydrogen peroxide-bound form of Prx has not been elucidated. In this paper, we report the crystal structure of Prx from Aeropyrum pernix K1 in the peroxide-bound form. The conformation of the polypeptide chain is the same as that in the reduced apo-form. The hydrogen peroxide molecule is in close contact with the peroxidatic Cys50 and the neighbouring Thr47 and Arg126 side chain atoms, as well as with the main chain nitrogen atoms of Val49 and Cys50. Bound peroxide was also observed in the mutant C50S, in which the peroxidatic cysteine was replaced by serine. Therefore, the sulphur atom of the peroxidatic cysteine is not essential for peroxide binding, although it enhances the binding affinity. Hydrogen peroxide binds to the protein so that it fills the active site pocket. This study provides insight into the early stage of the Prx reaction.
PDB ID: 3A2XDownload
MMDB ID: 77618
PDB Deposition Date: 2009/6/4
Updated in MMDB: 2012/10
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 3A2X: decameric; determined by author and by software (PISA)
Molecular Components in 3A2X
Label Count Molecule
Proteins (10 molecules)
10
Probable Peroxiredoxin
Molecule annotation
Chemicals (10 molecules)
1
10
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.