2ZKO: Structural Basis For Dsrna Recognition By Ns1 Protein Of Human Influenza Virus A

Influenza A viruses are important human pathogens causing periodic pandemic threats. Nonstructural protein 1 (NS1) protein of influenza A virus (NS1A) shields the virus against host defense. Here, we report the crystal structure of NS1A RNA-binding domain (RBD) bound to a double-stranded RNA (dsRNA) at 1.7A. NS1A RBD forms a homodimer to recognize the major groove of A-form dsRNA in a length-independent mode by its conserved concave surface formed by dimeric anti-parallel alpha-helices. dsRNA is anchored by a pair of invariable arginines (Arg38) from both monomers by extensive hydrogen bonds. In accordance with the structural observation, isothermal titration calorimetry assay shows that the unique Arg38-Arg38 pair and two Arg35-Arg46 pairs are crucial for dsRNA binding, and that Ser42 and Thr49 are also important for dsRNA binding. Agrobacterium co-infiltration assay further supports that the unique Arg38 pair plays important roles in dsRNA binding in vivo.Cell Research (2009) 19:187-195. doi: 10.1038/cr.2008.288; published online 23 September 2008.
PDB ID: 2ZKODownload
MMDB ID: 67308
PDB Deposition Date: 2008/3/26
Updated in MMDB: 2012/10
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 2ZKO: tetrameric; determined by author and by software (PISA)
Molecular Components in 2ZKO
Label Count Molecule
Proteins (2 molecules)
Non-structural Protein 1(Gene symbol: NS1)
Molecule annotation
Nucleotide(1 molecule)
RNA (5'- R(p*ap*gp*ap*cp*ap*gp*cp*ap*up*up*ap*up*gp*cp*up*gp*up*cp*up*up*u)- 3')
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB