2YEQ: Structure Of Phod

Citation:
Abstract
The PhoD family of extra-cytoplasmic phosphodiesterases are among the most commonly occurring bacterial phosphatases. The exemplars for this family are the PhoD protein of Bacillus subtilis and the phospholipase D of Streptomyces chromofuscus. We present the crystal structure of B. subtilis PhoD. PhoD is most closely related to purple acid phosphatases (PAPs) with both types of enzyme containing a tyrosinate-ligated Fe(3+) ion. However, the PhoD active site diverges from that found in PAPs and uses two Ca(2+) ions instead of the single extra Fe(2+), Mn(2+), or Zn(2+) ion present in PAPs. The PhoD crystals contain a phosphate molecule that coordinates all three active site metal ions and that is proposed to represent a product complex. A C-terminal helix lies over the active site and controls access to the catalytic center. The structure of PhoD defines a new phosphatase active site architecture based on Fe(3+) and Ca(2+) ions.
PDB ID: 2YEQDownload
MMDB ID: 98779
PDB Deposition Date: 2011/3/30
Updated in MMDB: 2014/11
Experimental Method:
x-ray diffraction
Resolution: 1.93  Å
Source Organism:
Similar Structures:
Biological Unit for 2YEQ: monomeric; determined by author
Molecular Components in 2YEQ
Label Count Molecule
Protein (1 molecule)
1
Alkaline Phosphatase D(Gene symbol: phoD)
Molecule annotation
Chemicals (45 molecules)
1
16
2
21
3
4
4
1
5
2
6
1
* Click molecule labels to explore molecular sequence information.

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