2YA3: Structure Of The Regulatory Fragment Of Mammalian Ampk In Complex With Coumarin Adp

Citation:
Abstract
The heterotrimeric AMP-activated protein kinase (AMPK) has a key role in regulating cellular energy metabolism; in response to a fall in intracellular ATP levels it activates energy-producing pathways and inhibits energy-consuming processes. AMPK has been implicated in a number of diseases related to energy metabolism including type 2 diabetes, obesity and, most recently, cancer. AMPK is converted from an inactive form to a catalytically competent form by phosphorylation of the activation loop within the kinase domain: AMP binding to the gamma-regulatory domain promotes phosphorylation by the upstream kinase, protects the enzyme against dephosphorylation, as well as causing allosteric activation. Here we show that ADP binding to just one of the two exchangeable AXP (AMP/ADP/ATP) binding sites on the regulatory domain protects the enzyme from dephosphorylation, although it does not lead to allosteric activation. Our studies show that active mammalian AMPK displays significantly tighter binding to ADP than to Mg-ATP, explaining how the enzyme is regulated under physiological conditions where the concentration of Mg-ATP is higher than that of ADP and much higher than that of AMP. We have determined the crystal structure of an active AMPK complex. The structure shows how the activation loop of the kinase domain is stabilized by the regulatory domain and how the kinase linker region interacts with the regulatory nucleotide-binding site that mediates protection against dephosphorylation. From our biochemical and structural data we develop a model for how the energy status of a cell regulates AMPK activity.
PDB ID: 2YA3Download
MMDB ID: 89164
PDB Deposition Date: 2011/2/17
Updated in MMDB: 2011/05
Experimental Method:
x-ray diffraction
Resolution: 2.5  Å
Source Organism:
Rattus norvegicus
Similar Structures:
Biological Unit for 2YA3: trimeric; determined by author and by software (PISA)
Molecular Components in 2YA3
Label Count Molecule
Proteins (3 molecules)
1
5'-amp-activated Protein Kinase Catalytic Subunit Alpha-1(Gene symbol: Prkaa1)
Molecule annotation
1
5'-amp-activated Protein Kinase Subunit Beta-2(Gene symbol: PRKAB2)
Molecule annotation
1
5'-amp-activated Protein Kinase Subunit Gamma-1(Gene symbol: Prkag1)
Molecule annotation
Chemicals (3 molecules)
1
2
2
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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