2Y4Q: Solution structure of the EF-hand domain of Human Polycystin 2

Autosomal dominant polycystic kidney disease (ADPKD) affects over 1:1000 of the worldwide population and is caused by mutations in two genes, PKD1 and PKD2. PKD2 encodes a 968-amino acid membrane spanning protein, Polycystin-2 (PC-2), which is a member of the TRP ion channel family. The C-terminal cytoplasmic tail contains an EF-hand motif followed by a short coiled-coil domain. We have determined the structure of the EF-hand region of PC-2 using NMR spectroscopy. The use of different boundaries, compared with those used in previous studies, have enabled us to determine a high resolution structure and show that the EF hand motif forms a standard calcium-binding pocket. The affinity of this pocket for calcium has been measured and mutants that both decrease and increase its affinity for the metal ion have been created.
PDB ID: 2Y4QDownload
MMDB ID: 95844
PDB Deposition Date: 2011/1/7
Updated in MMDB: 2011/12
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2Y4Q: monomeric; determined by author
Molecular Components in 2Y4Q
Label Count Molecule
Protein (1 molecule)
Polycystin-2(Gene symbol: PKD2)
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

Citing MMDB