2XS8: Crystal Structure Of Alix In Complex With The Sivagmtan-1 Aydparkll Late Domain

Retroviral Gag proteins contain short late-domain motifs that recruit cellular ESCRT pathway proteins to facilitate virus budding. ALIX-binding late domains often contain the core consensus sequence YPX(n)L (where X(n) can vary in sequence and length). However, some simian immunodeficiency virus (SIV) Gag proteins lack this consensus sequence, yet still bind ALIX. We mapped divergent, ALIX-binding late domains within the p6(Gag) proteins of SIV(mac239) ((40)SREKPYKEVTEDLLHLNSLF(59)) and SIV(agmTan-1) ((24)AAGAYDPARKLLEQYAKK(41)). Crystal structures revealed that anchoring tyrosines (in lightface) and nearby hydrophobic residues (underlined) contact the ALIX V domain, revealing how lentiviruses employ a diverse family of late-domain sequences to bind ALIX and promote virus budding.
PDB ID: 2XS8Download
MMDB ID: 85871
PDB Deposition Date: 2010/9/24
Updated in MMDB: 2010/12
Experimental Method:
x-ray diffraction
Resolution: 2.5  Å
Source Organism:
Simian immunodeficiency virus
Similar Structures:
Biological Unit for 2XS8: dimeric; determined by author and by software (PISA)
Molecular Components in 2XS8
Label Count Molecule
Proteins (2 molecules)
Programmed Cell Death 6-interacting Protein(Gene symbol: PDCD6IP)
Molecule annotation
Sivagmtan-1 GAG P6
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB