2XQD: The Structure Of Ef-Tu And Aminoacyl-Trna Bound To The 70s Ribosome With A Gtp Analog

Citation:
Abstract
Protein synthesis requires several guanosine triphosphatase (GTPase) factors, including elongation factor Tu (EF-Tu), which delivers aminoacyl-transfer RNAs (tRNAs) to the ribosome. To understand how the ribosome triggers GTP hydrolysis in translational GTPases, we have determined the crystal structure of EF-Tu and aminoacyl-tRNA bound to the ribosome with a GTP analog, to 3.2 angstrom resolution. EF-Tu is in its active conformation, the switch I loop is ordered, and the catalytic histidine is coordinating the nucleophilic water in position for inline attack on the gamma-phosphate of GTP. This activated conformation is due to a critical and conserved interaction of the histidine with A2662 of the sarcin-ricin loop of the 23S ribosomal RNA. The structure suggests a universal mechanism for GTPase activation and hydrolysis in translational GTPases on the ribosome.
MMDB ID: 99581
PDB Deposition Date: 2010/9/2
Updated in MMDB: 2012/08
Experimental Method:
x-ray diffraction
Resolution: 3.1  Å
Source Organism:
Thermus thermophilus HB8
Similar Structures:
Merged PDB IDs:2XQD 2XQE
Asymmetric Unit for 2XQD: 59-meric
Molecular Components in 2XQD
Label Count Molecule
Proteins (52 molecules)
1
30S Ribosomal Protein S2
Molecule annotation
1
30S Ribosomal Protein S3
Molecule annotation
1
30S Ribosomal Protein S4
Molecule annotation
1
30S Ribosomal Protein S5
Molecule annotation
1
30S Ribosomal Protein S6
Molecule annotation
1
30S Ribosomal Protein S7
Molecule annotation
1
30S Ribosomal Protein S8
Molecule annotation
1
30S Ribosomal Protein S9
Molecule annotation
1
30S Ribosomal Protein S10
Molecule annotation
1
30S Ribosomal Protein S11
Molecule annotation
1
30S Ribosomal Protein S12
Molecule annotation
1
30S Ribosomal Protein S13
Molecule annotation
1
30S Ribosomal Protein S14
Molecule annotation
1
30S Ribosomal Protein S15
Molecule annotation
1
30S Ribosomal Protein S16
Molecule annotation
1
30S Ribosomal Protein S17
Molecule annotation
1
30S Ribosomal Protein S18
Molecule annotation
1
30S Ribosomal Protein S19
Molecule annotation
1
30S Ribosomal Protein S20
Molecule annotation
1
30S Ribosomal Protein THX
Molecule annotation
1
Elongation Factor TU
Molecule annotation
1
50S Ribosomal Protein L27
Molecule annotation
1
50S Ribosomal Protein L28
Molecule annotation
1
50S Ribosomal Protein L29
Molecule annotation
1
50S Ribosomal Protein L30
Molecule annotation
1
50S Ribosomal Protein L31
Molecule annotation
1
50S Ribosomal Protein L32
Molecule annotation
1
50S Ribosomal Protein L33
Molecule annotation
1
50S Ribosomal Protein L34
Molecule annotation
1
50S Ribosomal Protein L35
Molecule annotation
1
50S Ribosomal Protein L36
Molecule annotation
1
50S Ribosomal Protein L1
Molecule annotation
1
50S Ribosomal Protein L2
Molecule annotation
1
50S Ribosomal Protein L3
Molecule annotation
1
50S Ribosomal Protein L4
Molecule annotation
1
50S Ribosomal Protein L5
Molecule annotation
1
50S Ribosomal Protein L6
Molecule annotation
1
50S Ribosomal Protein L10
Molecule annotation
1
50S Ribosomal Protein L11
Molecule annotation
1
50S Ribosomal Protein L13
Molecule annotation
1
50S Ribosomal Protein L14
Molecule annotation
1
50S Ribosomal Protein L15
Molecule annotation
1
50S Ribosomal Protein L16
Molecule annotation
1
50S Ribosomal Protein L17
Molecule annotation
1
50S Ribosomal Protein L18
Molecule annotation
1
50S Ribosomal Protein L19
Molecule annotation
1
50S Ribosomal Protein L20
Molecule annotation
1
50S Ribosomal Protein L21
Molecule annotation
1
50S Ribosomal Protein L22
Molecule annotation
1
50S Ribosomal Protein L23
Molecule annotation
1
50S Ribosomal Protein L24
Molecule annotation
1
50S Ribosomal Protein L25
Molecule annotation
Nucleotides(6 molecules)
1
16S rRNA
Molecule annotation
2
E-site tRNA PHE or P-site tRNA PHE
Molecule annotation
1
mRNA
Molecule annotation
1
A-site tRNA G24a Trp-trna TRP
Molecule annotation
1
23S Ribosomal RNA
Molecule annotation
1
5S Ribosomal RNA
Molecule annotation
Chemicals (7 molecules)
1
4
Molecule information is not avaliable.
2
1
Molecule information is not avaliable.
3
1
Molecule information is not avaliable.
4
1
Molecule information is not avaliable.
* Click molecule labels to explore molecular sequence information.

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