2XND: Crystal Structure Of Bovine F1-C8 Sub-Complex Of Atp Synthase

Citation:
Abstract
The catalytic domain of the F-ATPase in mitochondria protrudes into the matrix of the organelle, and is attached to the membrane domain by central and peripheral stalks. Energy for the synthesis of ATP from ADP and phosphate is provided by the transmembrane proton-motive-force across the inner membrane, generated by respiration. The proton-motive force is coupled mechanically to ATP synthesis by the rotation at about 100 times per second of the central stalk and an attached ring of c-subunits in the membrane domain. Each c-subunit carries a glutamate exposed around the midpoint of the membrane on the external surface of the ring. The rotation is generated by protonation and deprotonation successively of each glutamate. Each 360 degrees rotation produces three ATP molecules, and requires the translocation of one proton per glutamate by each c-subunit in the ring. In fungi, eubacteria, and plant chloroplasts, ring sizes of c(10)-c(15) subunits have been observed, implying that these enzymes need 3.3-5 protons to make each ATP, but until now no higher eukaryote has been examined. As shown here in the structure of the bovine F(1)-c-ring complex, the c-ring has eight c-subunits. As the sequences of c-subunits are identical throughout almost all vertebrates and are highly conserved in invertebrates, their F-ATPases probably contain c(8)-rings also. Therefore, in about 50,000 vertebrate species, and probably in many or all of the two million invertebrate species, 2.7 protons are required by the F-ATPase to make each ATP molecule.
PDB ID: 2XNDDownload
MMDB ID: 84772
PDB Deposition Date: 2010/8/2
Updated in MMDB: 2011/11
Experimental Method:
x-ray diffraction
Resolution: 3.5  Å
Source Organism:
Similar Structures:
Biological Unit for 2XND: heptadecameric; determined by author and by software (PISA)
Molecular Components in 2XND
Label Count Molecule
Proteins (17 molecules)
3
ATP Synthase Subunit Alpha, Mitochondrial(Gene symbol: ATP5F1A)
Molecule annotation
3
ATP Synthase Subunit Beta, Mitochondrial(Gene symbol: ATP5F1B)
Molecule annotation
1
ATP Synthase Subunit Gamma, Mitochondrial(Gene symbol: ATP5F1C)
Molecule annotation
1
ATP Synthase Subunit Delta, Mitochondrial(Gene symbol: ATP5F1D)
Molecule annotation
1
ATP Synthase Subunit Epsilon, Mitochondrial(Gene symbol: ATP5F1E)
Molecule annotation
8
ATP Synthase Lipid-binding Protein, Mitochondrial(Gene symbol: ATP5MC1)
Molecule annotation
Chemicals (12 molecules)
1
5
2
5
3
1
4
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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