2XGT: Asparaginyl-Trna Synthetase From Brugia Malayi Complexed With The Sulphamoyl Analogue Of Asparaginyl-Adenylate

Citation:
Abstract
Aminoacyl-tRNA synthetases are validated molecular targets for anti-infective drug discovery because of their essentiality in protein synthesis. Thanks to genome sequencing, it is now possible to systematically study aminoacyl-tRNA synthetases from human eukaryotic parasites as putative targets for novel drug discovery. As part of a program targeting class IIb asparaginyl-tRNA synthetases (AsnRS) from the parasitic nematode Brugia malayi for anti-filarial drugs, we report the complete structure of a eukaryotic AsnRS. Metazoan and fungal AsnRS differ from their bacterial homologues by the addition of a conserved N-terminal extension of about 110 residues whose structure we have determined by solution NMR for the B. malayi enzyme. In addition, we solved by X-ray crystallography a series of structures of the catalytically active N-terminally truncated enzyme (residues 112-548), allowing the structural basis for the mechanism of asparagine activation to be elucidated. The N-terminal domain contains a structured region with a novel fold featuring a lysine-rich helix that is shown by NMR to interact with tRNA. This is connected by an unstructured tether to the remainder of the enzyme, which is highly similar to the known structure of bacterial AsnRS. These data enable a model of the complete AsnRS-tRNA complex to be constructed.
PDB ID: 2XGTDownload
MMDB ID: 87280
PDB Deposition Date: 2010/6/7
Updated in MMDB: 2010/12
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 2XGT: dimeric; determined by author and by software (PISA)
Molecular Components in 2XGT
Label Count Molecule
Proteins (2 molecules)
2
Asparaginyl-trna Synthetase, Cytoplasmic
Molecule annotation
Chemicals (5 molecules)
1
1
2
2
3
2
* Click molecule labels to explore molecular sequence information.

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