2X9P: X-Ray Structure Of The Substrate-Free Cytochrome P450 Pimd - A Polyene Macrolide Antibiotic Pimaricin Epoxidase

Citation:
Abstract
We present the X-ray structure of PimD, both substrate-free and in complex with 4,5-desepoxypimaricin. PimD is a cytochrome P450 monooxygenase with native epoxidase activity that is critical in the biosynthesis of the polyene macrolide antibiotic pimaricin. Intervention in this secondary metabolic pathway could advance the development of drugs with improved pharmacologic properties. Epoxidation by P450 typically includes formation of a charge-transfer complex between an oxoferryl pi-cation radical species (Compound I) and the olefin pi-bond as the initial intermediate. Catalytic and structural evidence presented here suggest that epoxidation of 4,5-desepoxypimaricin proceeds via a hydroperoxoferric intermediate (Compound 0). The oxygen atom of Compound 0 distal to the heme iron may insert into the double bond of the substrate to make an epoxide ring. Stereoelectronic features of the putative transition state suggest substrate-assisted proton delivery.
PDB ID: 2X9PDownload
MMDB ID: 83776
PDB Deposition Date: 2010/3/23
Updated in MMDB: 2011/10
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Similar Structures:
Biological Unit for 2X9P: monomeric; determined by software (PISA)
Molecular Components in 2X9P
Label Count Molecule
Protein (1 molecule)
1
Pimd Protein
Molecule annotation
Chemicals (4 molecules)
1
1
2
3
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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