2X6A: Potassium Channel From Magnetospirillum Magnetotacticum

Citation:
Abstract
Potassium channels embedded in cell membranes employ gates to regulate K+ current. While a specific constriction in the permeation pathway has historically been implicated in gating, recent reports suggest that the signature ion selectivity filter located in the outer membrane leaflet may be equally important. Inwardly rectifying K+ channels also control the directionality of flow, using intracellular polyamines to stem ion efflux by a valve-like action. This study presents crystallographic evidence of interdependent gates in the conduction pathway and reveals the mechanism of polyamine block. Reorientation of the intracellular domains, concomitant with activation, instigates polyamine release from intracellular binding sites to block the permeation pathway. Conformational adjustments of the slide helices, achieved by rotation of the cytoplasmic assembly relative to the pore, are directly correlated to the ion configuration in the selectivity filter. Ion redistribution occurs irrespective of the constriction, suggesting a more expansive role of the selectivity filter in gating than previously appreciated.
PDB ID: 2X6ADownload
MMDB ID: 82890
PDB Deposition Date: 2010/2/15
Updated in MMDB: 2012/06
Experimental Method:
x-ray diffraction
Resolution: 3.1  Å
Source Organism:
Similar Structures:
Biological Unit for 2X6A: tetrameric; determined by author and by software (PISA)
Molecular Components in 2X6A
Label Count Molecule
Proteins (4 molecules)
4
Atp-sensitive Inward Rectifier Potassium Channel 10
Molecule annotation
Chemicals (7 molecules)
1
4
2
3
* Click molecule labels to explore molecular sequence information.

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