2X2Q: Crystal Structure Of An 'all Locked' Lna Duplex At 1.9 Angstrom Resolution

Citation:
Abstract
'Locked nucleic acids' (LNAs) are known to introduce enhanced bio- and thermostability into natural nucleic acids rendering them powerful tools for diagnostic and therapeutic applications. We present the 1.9 A X-ray structure of an 'all LNA' duplex containing exclusively modified beta-D-2'-O-4'C-methylene ribofuranose nucleotides. The helix illustrates a new type of nucleic acid geometry that contributes to the understanding of the enhanced thermostability of LNA duplexes. A notable decrease of several local and overall helical parameters like twist, roll and propeller twist influence the structure of the LNA helix and result in a widening of the major groove, a decrease in helical winding and an enlarged helical pitch. A detailed structural comparison to the previously solved RNA crystal structure with the corresponding base pair sequence underlines the differences in conformation. The surrounding water network of the RNA and the LNA helix shows a similar hydration pattern.
PDB ID: 2X2QDownload
MMDB ID: 88618
PDB Deposition Date: 2010/1/15
Updated in MMDB: 2017/07
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Biological Unit for 2X2Q: trimeric; determined by software (PISA)
Molecular Components in 2X2Q
Label Count Molecule
Nucleotides(2 molecules)
1
Locked Nucleic Acid Derived From tRNA SER Acceptor Stem Microhelix
Molecule annotation
2
Locked Nucleic Acid Derived From tRNA SER Acceptor Stem Microhelix
Molecule annotation
Chemicals (3 molecules)
1
1
2
1
3
1
* Click molecule labels to explore molecular sequence information.

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