2WWL: E.Coli 70s Ribosome Stalled During Translation Of Tnac Leader Peptide

Citation:
Abstract
Expression of the Escherichia coli tryptophanase operon depends on ribosome stalling during translation of the upstream TnaC leader peptide, a process for which interactions between the TnaC nascent chain and the ribosomal exit tunnel are critical. We determined a 5.8 angstrom-resolution cryo-electron microscopy and single-particle reconstruction of a ribosome stalled during translation of the tnaC leader gene. The nascent chain was extended within the exit tunnel, making contacts with ribosomal components at distinct sites. Upon stalling, two conserved residues within the peptidyltransferase center adopted conformations that preclude binding of release factors. We propose a model whereby interactions within the tunnel are relayed to the peptidyltransferase center to inhibit translation. Moreover, we show that nascent chains adopt distinct conformations within the ribosomal exit tunnel.
MMDB ID: 99577
PDB Deposition Date: 2009/10/26
Updated in MMDB: 2012/08
Experimental Method:
electron microscopy
Resolution: 5.8  Å
Source Organism:
Escherichia coli
Similar Structures:
Merged PDB IDs:2WWL 2WWQ
Biological Unit for 2WWL: 59-meric
Molecular Components in 2WWL
Label Count Molecule
Proteins (52 molecules)
1
30S Ribosomal Protein S2
Molecule annotation
1
30S Ribosomal Protein S3
Molecule annotation
1
30S Ribosomal Protein S4
Molecule annotation
1
30S Ribosomal Protein S5
Molecule annotation
1
30S Ribosomal Protein S6
Molecule annotation
1
30S Ribosomal Protein S7
Molecule annotation
1
30S Ribosomal Protein S8
Molecule annotation
1
30S Ribosomal Protein S9
Molecule annotation
1
30S Ribosomal Protein S10
Molecule annotation
1
30S Ribosomal Protein S11
Molecule annotation
1
30S Ribosomal Protein S12
Molecule annotation
1
30S Ribosomal Protein S13
Molecule annotation
1
30S Ribosomal Protein S14
Molecule annotation
1
30S Ribosomal Protein S15
Molecule annotation
1
30S Ribosomal Protein S16
Molecule annotation
1
30S Ribosomal Protein S17
Molecule annotation
1
30S Ribosomal Protein S18
Molecule annotation
1
30S Ribosomal Protein S19
Molecule annotation
1
30S Ribosomal Protein S20
Molecule annotation
1
30S Ribosomal Protein S21
Molecule annotation
2
Poly-ala Nascent Chain
Molecule annotation
1
50S Ribosomal Protein L1
Molecule annotation
1
50S Ribosomal Protein L2
Molecule annotation
1
50S Ribosomal Protein L3
Molecule annotation
1
50S Ribosomal Protein L4
Molecule annotation
1
50S Ribosomal Protein L5
Molecule annotation
1
50S Ribosomal Protein L6
Molecule annotation
1
50S Ribosomal Protein L9
Molecule annotation
1
50S Ribosomal Protein L11
Molecule annotation
1
50S Ribosomal Protein L13
Molecule annotation
1
50S Ribosomal Protein L14
Molecule annotation
1
50S Ribosomal Protein L15
Molecule annotation
1
50S Ribosomal Protein L16
Molecule annotation
1
50S Ribosomal Protein L17
Molecule annotation
1
50S Ribosomal Protein L18
Molecule annotation
1
50S Ribosomal Protein L19
Molecule annotation
1
50S Ribosomal Protein L20
Molecule annotation
1
50S Ribosomal Protein L21
Molecule annotation
1
50S Ribosomal Protein L22
Molecule annotation
1
50S Ribosomal Protein L23
Molecule annotation
1
50S Ribosomal Protein L24
Molecule annotation
1
50S Ribosomal Protein L25
Molecule annotation
1
50S Ribosomal Protein L27
Molecule annotation
1
50S Ribosomal Protein L28
Molecule annotation
1
50S Ribosomal Protein L29
Molecule annotation
1
50S Ribosomal Protein L30
Molecule annotation
1
50S Ribosomal Protein L32
Molecule annotation
1
50S Ribosomal Protein L33
Molecule annotation
1
50S Ribosomal Protein L34
Molecule annotation
1
50S Ribosomal Protein L35
Molecule annotation
1
50S Ribosomal Protein L36
Molecule annotation
Nucleotides(5 molecules)
1
16S Ribosomal RNA
Molecule annotation
2
mRNA
Molecule annotation
2
P-site tRNA
Molecule annotation
1
23S Ribosomal RNA
Molecule annotation
1
5S Ribosomal RNA
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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