2WJM: Lipidic Sponge Phase Crystal Structure Of The Photosynthetic Reaction Centre From Blastochloris Viridis (low Dose)

Citation:
Abstract
Membrane proteins are embedded in a lipid bilayer and maintain strong interactions with lipid molecules. Tightly bound lipids are responsible for vertical positioning and integration of proteins in the membrane and for assembly of multisubunit complexes and occasionally act as substrates. In this work we present the lipidic sponge phase crystal structure of the reaction center from Blastochloris viridis to 1.86 A, which reveals lipid molecules interacting with the protein surface. A diacylglycerol molecule is bound, through a thioether bond, to the N-terminus of the tetraheme cytochrome c subunit. From the electron density recovered at the Q(B) site and the observed change in recombination kinetics in lipidic sponge phase-grown crystals, the mobile ubiquinone appears to be displaced by a monoolein molecule. A 36 A long electron density feature is observed at the interface of transmembrane helices belonging to the H- and M-subunits, probably arising from an unidentified lipid.
PDB ID: 2WJMDownload
MMDB ID: 76801
PDB Deposition Date: 2009/5/27
Updated in MMDB: 2011/12
Experimental Method:
x-ray diffraction
Resolution: 1.95  Å
Source Organism:
Similar Structures:
Biological Unit for 2WJM: tetrameric; determined by author and by software (PISA)
Molecular Components in 2WJM
Label Count Molecule
Proteins (4 molecules)
1
Photosynthetic Reaction Center Cytochrome C Subunit
Molecule annotation
1
Reaction Center Protein H Chain
Molecule annotation
1
Reaction Center Protein L Chain
Molecule annotation
1
Reaction Center Protein M Chain
Molecule annotation
Chemicals (19 molecules)
1
4
2
1
3
4
4
2
5
3
6
1
7
1
8
1
9
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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