2WIU: Mercury-Modified Bacterial Persistence Regulator Hipba

Bacterial persistence is the ability of individual cells to randomly enter a period of dormancy during which the cells are protected against antibiotics. In Escherichia coli, persistence is regulated by the activity of a protein kinase HipA and its DNA-binding partner HipB, which is a strong inhibitor of both HipA activity and hip operon transcription. The crystal structure of the HipBA complex was solved by application of the SAD technique to a mercury derivative. In this article, the fortuitous and interesting effect of mercury soaks on the native HipBA crystals is discussed as well as the intriguing tryptophan-binding pocket found on the HipA surface. A HipA-regulation model is also proposed that is consistent with the available structural and biochemical data.
PDB ID: 2WIUDownload
MMDB ID: 75552
PDB Deposition Date: 2009/5/17
Updated in MMDB: 2009/07
Experimental Method:
x-ray diffraction
Resolution: 2.35  Å
Source Organism:
Similar Structures:
Biological Unit for 2WIU: tetrameric; determined by author and by software (PISA)
Molecular Components in 2WIU
Label Count Molecule
Proteins (4 molecules)
Protein Hipa(Gene symbol: hipA)
Molecule annotation
Hth-type Transcriptional Regulator Hipb(Gene symbol: hipB)
Molecule annotation
Chemicals (17 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB