2VE7: Crystal Structure Of A Bonsai Version Of The Human Ndc80 Complex

Kinetochores are proteinaceous assemblies that mediate the interaction of chromosomes with the mitotic spindle. The 180 kDa Ndc80 complex is a direct point of contact between kinetochores and microtubules. Its four subunits contain coiled coils and form an elongated rod structure with functional globular domains at either end. We crystallized an engineered "bonsai" Ndc80 complex containing a shortened rod domain but retaining the globular domains required for kinetochore localization and microtubule binding. The structure reveals a microtubule-binding interface containing a pair of tightly interacting calponin-homology (CH) domains with a previously unknown arrangement. The interaction with microtubules is cooperative and predominantly electrostatic. It involves positive charges in the CH domains and in the N-terminal tail of the Ndc80 subunit and negative charges in tubulin C-terminal tails and is regulated by the Aurora B kinase. We discuss our results with reference to current models of kinetochore-microtubule attachment and centromere organization.
PDB ID: 2VE7Download
MMDB ID: 64402
PDB Deposition Date: 2007/10/17
Updated in MMDB: 2012/09
Experimental Method:
x-ray diffraction
Resolution: 2.88  Å
Source Organism:
Similar Structures:
Biological Unit for 2VE7: dimeric; determined by author and by software (PISA)
Molecular Components in 2VE7
Label Count Molecule
Proteins (2 molecules)
Kinetochore Protein Hec1, Kinetochore Protein Spc25
Molecule annotation
Kinetochore Protein Nuf2, Kinetochore Protein Spc24
Molecule annotation
Chemicals (3 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB