2VBE: Tailspike Protein Of Bacteriophage Sf6

Citation:
Abstract
Sf6 belongs to the Podoviridae family of temperate bacteriophages that infect gram-negative bacteria by insertion of their double-stranded DNA. They attach to their hosts specifically via their tailspike proteins. The 1.25 A crystal structure of Shigella phage Sf6 tailspike protein (Sf6 TSP) reveals a conserved architecture with a central, right-handed beta helix. In the trimer of Sf6 TSP, the parallel beta helices form a left-handed, coiled-beta coil with a pitch of 340 A. The C-terminal domain consists of a beta sandwich reminiscent of viral capsid proteins. Further crystallographic and biochemical analyses show a Shigella cell wall O-antigen fragment to bind to an endorhamnosidase active site located between two beta-helix subunits each anchoring one catalytic carboxylate. The functionally and structurally related bacteriophage, P22 TSP, lacks sequence identity with Sf6 TSP and has its active sites on single subunits. Sf6 TSP may serve as an example for the evolution of different host specificities on a similar general architecture.
PDB ID: 2VBEDownload
MMDB ID: 63942
PDB Deposition Date: 2007/9/12
Updated in MMDB: 2011/05
Experimental Method:
x-ray diffraction
Resolution: 1.98  Å
Source Organism:
Similar Structures:
Biological Unit for 2VBE: trimeric; determined by author and by software (PQS)
Molecular Components in 2VBE
Label Count Molecule
Proteins (3 molecules)
3
Tailspike-protein
Molecule annotation
Chemicals (29 molecules)
1
3
2
2
3
18
4
6
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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