2RU6: The pure alternative state of ubiquitin

We present the nuclear Overhauser effect-based structure determination of the Q41N variant of ubiquitin at 2500 bar, where the alternatively folded N2 state is 97% populated. This allows us to characterize the structure of the "pure" N2 state of ubiquitin. The N2 state shows a substantial change in the orientation of strand beta5 compared to that of the normal folded N1 state, which matches the changes seen upon binding of ubiquitin to ubiquitin-activating enzyme E1. The recognition of E1 by ubiquitin is therefore best explained by conformational selection rather than induced-fit motion.
PDB ID: 2RU6Download
MMDB ID: 117363
PDB Deposition Date: 2013/12/4
Updated in MMDB: 2018/05
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2RU6: monomeric; determined by author
Molecular Components in 2RU6
Label Count Molecule
Protein (1 molecule)
Ubiquitin(Gene symbol: UBC)
Molecule annotation
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Citing MMDB