2RQK: NMR Solution Structure of Mesoderm Development (MESD) - closed conformation

Mesoderm development (MESD) is a 224 amino acid mouse protein that acts as a molecular chaperone for the low-density lipoprotein receptor (LDLR) family. Here, we provide evidence that the region 45-184 of MESD is essential and sufficient for this function and suggest a model for its mode of action. NMR studies reveal a beta-alpha-beta-beta-alpha-beta core domain with an alpha-helical N-terminal extension that interacts with the beta sheet in a dynamic manner. As a result, the structural ensemble contains open (active) and closed (inactive) forms, allowing for regulation of chaperone activity through substrate binding. The mutant W61R, which is lethal in Drosophila, adopts only the open state. The receptor motif recognized by MESD was identified by in vitro-binding studies. Furthermore, in vivo functional evidence for the relevance of the identified contact sites in MESD is provided.
PDB ID: 2RQKDownload
MMDB ID: 76170
PDB Deposition Date: 2009/8/6
Updated in MMDB: 2009/08
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2RQK: monomeric; determined by author
Molecular Components in 2RQK
Label Count Molecule
Protein (1 molecule)
Mesoderm Development Candidate 2(Gene symbol: Mesd)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB