2RO3: RDC-refined Solution Structure of the N-terminal DNA Recognition Domain of the Bacillus subtilis Transition-state Regulator Abh

Understanding the DNA recognition and binding by the AbrB-like family of transcriptional regulators is of significant interest since these proteins enable bacteria to elicit the appropriate response to diverse environmental stimuli. Although these "transition-state regulator" proteins have been well characterized at the genetic level, the general and specific mechanisms of DNA binding remain elusive. We present RDC-refined NMR solution structures and dynamic properties of the DNA-binding domains of three Bacillus subtilis transition-state regulators: AbrB, Abh, and SpoVT. We combined previously investigated DNase I footprinting, DNA methylation, gel-shift assays, and mutagenic and NMR studies to generate a structural model of the complex between AbrBN(55) and its cognate promoter, abrB8. These investigations have enabled us to generate a model for the specific nature of the transition-state regulator-DNA interaction, a structure that has remained elusive thus far.
PDB ID: 2RO3Download
MMDB ID: 67839
PDB Deposition Date: 2008/3/8
Updated in MMDB: 2008/11
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2RO3: dimeric; determined by author
Molecular Components in 2RO3
Label Count Molecule
Proteins (2 molecules)
Putative Transition State Regulator ABH(Gene symbol: abh)
Molecule annotation
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Citing MMDB