2RHS: Phers From Staphylococcus Haemolyticus- Rational Protein Engineering And Inhibitor Studies

Citation:
Abstract
In this article, we describe for the first time the high-resolution crystal structure of a phenylalanine tRNA synthetase from the pathogenic bacterium Staphylococcus haemolyticus. We demonstrate the subtle yet important structural differences between this enzyme and the previously described Thermus thermophilus ortholog. We also explain the structure-activity relationship of several recently reported inhibitors. The native enzyme crystals were of poor quality--they only diffracted X-rays to 3-5A resolution. Therefore, we have executed a rational surface mutagenesis strategy that has yielded crystals of this 2300-amino acid multidomain protein, diffracting to 2A or better. This methodology is discussed and contrasted with the more traditional domain truncation approach.
PDB ID: 2RHSDownload
MMDB ID: 60604
PDB Deposition Date: 2007/10/9
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.2  Å
Source Organism:
Similar Structures:
Biological Unit for 2RHS: tetrameric; determined by author and by software (PISA)
Molecular Components in 2RHS
Label Count Molecule
Proteins (4 molecules)
2
Phenylalanyl-trna Synthetase Alpha Chain(Gene symbol: SH_RS08890)
Molecule annotation
2
Phenylalanyl-trna Synthetase Beta Chain(Gene symbol: SH_RS08885)
Molecule annotation
Chemicals (5 molecules)
1
2
2
2
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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