2RFO: Crystral Structure Of The Nucleoporin Nic96

Citation:
Abstract
Nic96 is a conserved nucleoporin that recruits the Nsp1-Nup49-Nup57 complex, a module with Phe-Gly (FG) repeats, to the central transport channel of the nuclear pore complex (NPC). Nic96 binds the Nsp1 complex via its N domain and assembles into the NPC framework via its central and C domain. Here, we report the crystal structure of a large structural nucleoporin, Nic96 without its N domain (Nic96DeltaN). Nic96DeltaN is composed of three domains and is a straight molecule that--although almost entirely helical--exhibits strong deviations from the predicted alpha-solenoid fold. The missing N domain projects midway from the Nic96 molecule, indicating how the Nsp1 complex might be located with respect to the rod-like Nic96. Notably, Nic96DeltaN binds in vitro to FG repeats of the Nsp1 complex. These data suggest a model of how Nic96 could organize a transport module with coiled-coil domains and FG repeats in the central pore channel.
PDB ID: 2RFODownload
MMDB ID: 62115
PDB Deposition Date: 2007/10/1
Updated in MMDB: 2012/12
Experimental Method:
x-ray diffraction
Resolution: 2.6  Å
Source Organism:
Similar Structures:
Biological Unit for 2RFO: monomeric; determined by author and by software (PISA)
Molecular Components in 2RFO
Label Count Molecule
Protein (1 molecule)
1
Nucleoporin Nic96(Gene symbol: NIC96)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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