2RFK: Substrate Rna Positioning In The Archaeal HACA Ribonucleoprotein Complex

Citation:
Abstract
The most complex RNA pseudouridylases are H/ACA ribonucleoprotein particles, which use a guide RNA for substrate capture and four proteins (Cbf5, Nop10, Gar1 and L7Ae/NHP2) for substrate modification. Here we report the three-dimensional structure of a catalytically deficient archaeal enzyme complex (including the guide RNA and three of the four essential proteins) bound to a substrate RNA. Extensive interactions of Cbf5 with one guide-substrate helix and a guide RNA stem shape the forked guide-substrate RNA complex structure and position the substrate in proximity of the Cbf5 catalytic center. Our structural and complementary fluorescence analyses also indicate that precise placement of the target uridine at the active site requires a conformation of the guide-substrate RNA duplex that is brought about by the previously identified concurrent interaction of the guide RNA with L7Ae and a composite Cbf5-Nop10 surface, and further identify a residue that is critical in this process.
PDB ID: 2RFKDownload
MMDB ID: 62244
PDB Deposition Date: 2007/9/30
Updated in MMDB: 2011/10
Experimental Method:
x-ray diffraction
Resolution: 2.87  Å
Source Organism:
synthetic construct
Similar Structures:
Biological Unit for 2RFK: hexameric; determined by author and by software (PISA)
Molecular Components in 2RFK
Label Count Molecule
Proteins (3 molecules)
1
Probable tRNA Pseudouridine Synthase B(Gene symbol: PF_RS09020)
Molecule annotation
1
Ribosome Biogenesis Protein Nop10(Gene symbol: PF_RS05730)
Molecule annotation
1
Small Nucleolar RNP Similar to Gar1
Molecule annotation
Nucleotides(3 molecules)
1
Guide RNA 1
Molecule annotation
1
Guide RNA 2
Molecule annotation
1
Target RNA
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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