2QJP: Crystal Structure Of Wild Type Rhodobacter Sphaeroides With Stigmatellin And Antimycin Inhibited

Citation:
Abstract
The cytochrome bc(1) complex (bc(1)) is a major contributor to the proton motive force across the membrane by coupling electron transfer to proton translocation. The crystal structures of wild type and mutant bc(1) complexes from the photosynthetic purple bacterium Rhodobacter sphaeroides (Rsbc(1)), stabilized with the quinol oxidation (Q(P)) site inhibitor stigmatellin alone or in combination with the quinone reduction (Q(N)) site inhibitor antimycin, were determined. The high quality electron density permitted assignments of a new metal-binding site to the cytochrome c(1) subunit and a number of lipid and detergent molecules. Structural differences between Rsbc(1) and its mitochondrial counterparts are mostly extra membranous and provide a basis for understanding the function of the predominantly longer sequences in the bacterial subunits. Functional implications for the bc(1) complex are derived from analyses of 10 independent molecules in various crystal forms and from comparisons with mitochondrial complexes.
PDB ID: 2QJPDownload
MMDB ID: 61519
PDB Deposition Date: 2007/7/8
Updated in MMDB: 2007/12
Experimental Method:
x-ray diffraction
Resolution: 2.6  Å
Source Organism:
Similar Structures:
Biological Unit for 2QJP: hexameric; determined by author and by software (PISA)
Molecular Components in 2QJP
Label Count Molecule
Proteins (6 molecules)
2
Cytochrome B
Molecule annotation
2
Cytochrome C1
Molecule annotation
2
Ubiquinol-cytochrome C Reductase Iron-sulfur Subunit
Molecule annotation
Chemicals (18 molecules)
1
6
2
2
3
2
4
2
5
2
6
2
7
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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