2QIJ: Hepatitis B Capsid Protein with an N-terminal extension modelled into 8.9 A data

Hepatitis B core (HBc) particles have been extensively exploited as carriers for foreign immunological epitopes in the development of multicomponent vaccines and diagnostic reagents. Crystals of the T = 4 HBc particle were grown in PEG 20,000, ammonium sulfate and various types of alcohols. A temperature jump from 277 or 283 to 290 K was found to enhance crystal growth. A crystal grown using MPD as a cryoprotectant diffracted X-rays to 7.7 A resolution and data were collected to 99.6% completeness at 8.9 A. The crystal belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 352.3, b = 465.5, c = 645.0 A. The electron-density map reveals a protrusion that is consistent with the N-terminus extending out from the surface of the capsid. The structure presented here supports the idea that N-terminal insertions can be exploited in the development of diagnostic reagents, multicomponent vaccines and delivery vehicles into mammalian cells.
PDB ID: 2QIJDownload
MMDB ID: 61190
PDB Deposition Date: 2007/7/4
Updated in MMDB: 2007/12
Experimental Method:
x-ray diffraction
Resolution: 8.9  Å
Source Organism:
Similar Structures:
Biological Unit for 2QIJ: 240-meric
Molecular Components in 2QIJ
Label Count Molecule
Proteins (240 molecules)
Core Antigen
Molecule annotation
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Citing MMDB