National Center for
2QCX: Crystal Structure Of Bacillus Subtilis Tena Y112f Mutant Complexed With Formyl Aminomethyl Pyrimidine
Bioorg. Chem. (2008) 36 p.29-32
TenA catalyzes the hydrolysis of 4-amino-5-aminomethyl-2-methylpyrimidine and participates in the salvage of base-degraded thiamin. Here, we describe mutagenesis of the active site of TenA guided by structures of the enzyme complexed to a substrate analog and to the product. Catalytic roles for each of the active site residues are identified and a mechanism for the reaction is described.