2PXV: Variant 6 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle

X-ray crystallography of biologically important RNA molecules has been hampered by technical challenges, including finding heavy-atom derivatives to obtain high-quality experimental phase information. Existing techniques have drawbacks, limiting the rate at which important new structures are solved. To address this, we have developed a reliable means to localize heavy atoms specifically to virtually any RNA. By solving the crystal structures of thirteen variants of the G*U wobble pair cation binding motif, we have identified a version that when inserted into an RNA helix introduces a high-occupancy cation binding site suitable for phasing. This "directed soaking" strategy can be integrated fully into existing RNA crystallography methods, potentially increasing the rate at which important structures are solved and facilitating routine solving of structures using Cu-Kalpha radiation. This method already has been used to solve several crystal structures.
PDB ID: 2PXVDownload
MMDB ID: 54109
PDB Deposition Date: 2007/5/14
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
Escherichia coli
Similar Structures:
Biological Unit for 2PXV: dimeric; determined by author
Molecular Components in 2PXV
Label Count Molecule
Protein (1 molecule)
Signal Recognition Particle Protein(Gene symbol: ffh)
Molecule annotation
Nucleotide(1 molecule)
4.5 S RNA
Molecule annotation
Chemicals (8 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB