2PS5: N225d Trichodiene Synthase: Complex With Mg And Pyrophosphate

Citation:
Abstract
Trichodiene synthase from Fusarium sporotrichioides contains two metal ion-binding motifs required for the cyclization of farnesyl diphosphate: the "aspartate-rich" motif D(100)DXX(D/E) that coordinates to Mg2+A and Mg2+C, and the "NSE/DTE" motif N(225)DXXSXXXE that chelates Mg2+B (boldface indicates metal ion ligands). Here, we report steady-state kinetic parameters, product array analyses, and X-ray crystal structures of trichodiene synthase mutants in which the fungal NSE motif is progressively converted into a plant-like DDXXTXXXE motif, resulting in a degradation in both steady-state kinetic parameters and product specificity. Each catalytically active mutant generates a different distribution of sesquiterpene products, and three newly detected sesquiterpenes are identified. In addition, the kinetic and structural properties of the Y295F mutant of trichodiene synthase were found to be similar to those of the wild-type enzyme, thereby ruling out a proposed role for Y295 in catalysis.
PDB ID: 2PS5Download
MMDB ID: 61344
PDB Deposition Date: 2007/5/4
Updated in MMDB: 2007/12
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Similar Structures:
Biological Unit for 2PS5: dimeric; determined by author and by software (PISA,PQS)
Molecular Components in 2PS5
Label Count Molecule
Proteins (2 molecules)
2
Trichodiene Synthase
Molecule annotation
Chemicals (7 molecules)
1
3
2
3
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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