2PJW: The Vps27/hse1 Complex IS a GAT Domain-based Scaffold for Ubiquitin- Dependent Sorting

The yeast Vps27/Hse1 complex and the homologous mammalian Hrs/STAM complex deliver ubiquitinated transmembrane proteins to the ESCRT endosomal-sorting pathway. The Vps27/Hse1 complex directly binds to ubiquitinated transmembrane proteins and recruits both ubiquitin ligases and deubiquitinating enzymes. We have solved the crystal structure of the core responsible for the assembly of the Vps27/Hse1 complex at 3.0 A resolution. The structure consists of two intertwined GAT domains, each consisting of two helices from one subunit and one from the other. The two GAT domains are connected by an antiparallel coiled coil, forming a 90 A-long barbell-like structure. This structure places the domains of Vps27 and Hse1 that recruit ubiquitinated cargo and deubiquitinating enzymes close to each other. Coarse-grained Monte Carlo simulations of the Vps27/Hse1 complex on a membrane show how the complex binds cooperatively to lipids and ubiquitinated membrane proteins and acts as a scaffold for ubiquitination reactions.
PDB ID: 2PJWDownload
MMDB ID: 46588
PDB Deposition Date: 2007/4/16
Updated in MMDB: 2017/10
Experimental Method:
x-ray diffraction
Resolution: 3.01  Å
Source Organism:
Similar Structures:
Biological Unit for 2PJW: dimeric; determined by author and by software (PISA)
Molecular Components in 2PJW
Label Count Molecule
Proteins (2 molecules)
Uncharacterized Protein Yhl002w(Gene symbol: HSE1)
Molecule annotation
Vacuolar Protein Sorting-associated Protein 27(Gene symbol: VPS27)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB