2P7C: Solution structure of the bacillus licheniformis BlaI monomeric form in complex with the blaP half-operator

Citation:
Abstract
In absence of beta-lactam antibiotics, BlaI and MecI homodimeric repressors negatively control the expression of genes involved in beta-lactam resistance in Bacillus licheniformis and in Staphylococcus aureus. Subsequently to beta-lactam presence, BlaI/MecI is inactivated by a single-point proteolysis that separates its N-terminal DNA-binding domain to its C-terminal domain responsible for its dimerization. Concomitantly to this proteolysis, the truncated repressor acquires a low affinity for its DNA target that explains the expression of the structural gene for resistance. To understand the loss of the high DNA affinity of the truncated repressor, we have determined the different dissociation constants of the system and solved the solution structure of the B. licheniformis monomeric repressor complexed to the semi-operating sequence OP1 of blaP (1/2OP1blaP) by using a de novo docking approach based on inter-molecular nuclear Overhauser effects and chemical-shift differences measured on each macromolecular partner. Although the N-terminal domain of the repressor is not subject to internal structural rearrangements upon DNA binding, the molecules adopt a tertiary conformation different from the crystallographic operator-repressor dimer complex, leading to a 30 degrees rotation of the monomer with respect to a central axis extended across the DNA. These results open new insights for the repression and induction mechanisms of bacterial resistance to beta-lactams.
PDB ID: 2P7CDownload
MMDB ID: 54023
PDB Deposition Date: 2007/3/20
Updated in MMDB: 2007/11
Experimental Method:
solution nmr
Source Organism:
Bacillus licheniformis
Similar Structures:
Molecular Components in 2P7C
Label Count Molecule
Protein (1 molecule)
1
Penicillinase Repressor
Molecule annotation
Nucleotides(2 molecules)
1
Strand 1 of Twelve Base-pair DNA
Molecule annotation
1
Strand 2 of Twelve Base-pair DNA
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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