2OQU: High Pressure Cryocooling Of Capillary Sample Cryoprotection And Diffraction Phasing At Long Wavelengths

Citation:
Abstract
Crystal cryocooling is usually employed to reduce radiation damage during X-ray crystallography. Recently, a high-pressure cryocooling method has been developed which results in excellent diffraction-quality crystals without the use of penetrative cryoprotectants. Three new developments of the method are presented here: (i) Xe-He high-pressure cryocooling for Xe SAD phasing, (ii) native sulfur SAD phasing and (iii) successful cryopreservation of crystals in thick-walled capillaries without additional cryoprotectants other than the native mother liquor. These developments may be useful for structural solution of proteins without the need for selenomethionine incorporation and for high-throughput protein crystallography.
PDB ID: 2OQUDownload
MMDB ID: 45531
PDB Deposition Date: 2007/2/1
Updated in MMDB: 2007/11
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 2OQU: monomeric; determined by author
Molecular Components in 2OQU
Label Count Molecule
Protein (1 molecule)
1
Elastase-1(Gene symbol: CELA1)
Molecule annotation
Chemicals (3 molecules)
1
1
2
1
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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