2NZ1: Viral Chemokine Binding Protein M3 From Murine Gammaherpesvirus68 In Complex With The Cc-Chemokine Ccl2MCP-1

Viruses have evolved a myriad of evasion strategies focused on undermining chemokine-mediated immune surveillance, exemplified by the mouse gamma-herpesvirus 68 M3 decoy receptor. Crystal structures of M3 in complex with C chemokine ligand 1/lymphotactin and CC chemokine ligand 2/monocyte chemoattractant protein 1 reveal that invariant chemokine features associated with G protein-coupled receptor binding are primarily recognized by the decoy C-terminal domain, whereas the N-terminal domain (NTD) reconfigures to engage divergent basic residue clusters on the surface of chemokines. Favorable electrostatic forces dramatically enhance the association kinetics of chemokine binding by M3, with a primary role ascribed to acidic NTD regions that effectively mimic glycosaminoglycan interactions. Thus, M3 employs two distinct mechanisms of chemical imitation to potently sequester chemokines, thereby inhibiting chemokine receptor binding events as well as the formation of chemotactic gradients necessary for directed leukocyte trafficking.
PDB ID: 2NZ1Download
MMDB ID: 61480
PDB Deposition Date: 2006/11/22
Updated in MMDB: 2007/12
Experimental Method:
x-ray diffraction
Resolution: 2.5  Å
Source Organism:
Murid gammaherpesvirus 4
Similar Structures:
Biological Unit for 2NZ1: tetrameric; determined by author and by software (PISA,PQS)
Molecular Components in 2NZ1
Label Count Molecule
Proteins (4 molecules)
Hypothetical Protein Gammahv.m3
Molecule annotation
Small Inducible Cytokine A2(Gene symbol: CCL2)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB