2MWN: Talin-F3 / RIAM N-terminal Peptide complex

The membrane localization and activation of cytoskeletal protein talin are key steps to initiate the integrin transmembrane receptors' activation, which mediates many cellular adhesive responses such as cell migration, spreading and proliferation. RIAM, a membrane anchor and small GTPase RAP1 effector, is known to bind to the C-terminal rod domain of talin (talin-R) and promote localizations of talin to the membrane. Through systematic mapping analysis, we find that RIAM also binds to the N-terminal head of talin (talin-H), a crucial domain involved in binding and activating integrins. We show that the RIAM binding to talin-H sterically occludes the binding of a talin-R domain that otherwise masks the integrin-binding site on talin-H. We further provide functional evidence that such RIAM-mediated steric unmasking of talin triggers integrin activation. Our findings thus uncover a novel role for RIAM in conformational regulation of talin during integrin activation and cell adhesion.
PDB ID: 2MWNDownload
MMDB ID: 125546
PDB Deposition Date: 2014/11/13
Updated in MMDB: 2014/12
Experimental Method:
solution nmr
Source Organism:
Homo sapiens
Similar Structures:
Biological Unit for 2MWN: dimeric; determined by author
Molecular Components in 2MWN
Label Count Molecule
Proteins (2 molecules)
Amyloid Beta A4 Precursor Protein-binding Family B Member 1-interacting Protein(Gene symbol: APBB1IP)
Molecule annotation
Talin-1(Gene symbol: TLN1)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB