2MW6: Structure of the bee venom toxin melittin with [(C5H5)Ru]+ fragment attached to the tryptophan residue

Melittin is a membrane-active peptide from bee venom with promising antimicrobial and anticancer activity. Herein we report on a simple and selective method for labeling of the tryptophan residue in melittin by the organometallic fragment [(C5 H5 )Ru](+) in aqueous solution and in air. Ruthenium coordination does not disturb the secondary structure of the peptide (as verified by 2D NMR spectroscopy), but changes the pattern of its intermolecular interactions resulting in an 11-fold decrease of hemolytic activity. The high stability of the organometallic conjugate allowed the establishment of the biodistribution of the labeled melittin in mice by inductively coupled plasma MS analysis of ruthenium.
PDB ID: 2MW6Download
MMDB ID: 130399
PDB Deposition Date: 2014/10/28
Updated in MMDB: 2015/07
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2MW6: monomeric; determined by author
Molecular Components in 2MW6
Label Count Molecule
Protein (1 molecule)
Melittin(Gene symbol: Melt)
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

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