2MJM: The solution NMR structure of the NLRC5 caspase recruitment domain (CARD)

The cytosolic nucleotide-binding domain and leucine-rich repeat-containing receptors (NLRs) are key sensors for bacterial and viral invaders and endogenous stress signals. NLRs contain a varying N-terminal effector domain that regulates the downstream signaling events upon its activation and determines the subclass to which a NLR member belongs. NLRC5 contains an unclassified N-terminal effector domain that has been reported to interact downstream with the tandem caspase recruitment domain (CARD) of retinoic acid-inducible gene I (RIG-I). Here we report the solution structure of the N-terminal effector domain of NLRC5 and in vitro interaction experiments with the tandem CARD of RIG-I. The N-terminal effector domain of NLRC5 adopts a six alpha-helix bundle with a general death fold, though it displays specific structural features that are strikingly different from the CARD. Notably, alpha-helix 3 is replaced by an ordered loop, and alpha-helix 1 is devoid of the characteristic interruption. Detailed structural alignments between the N-terminal effector domains of NLRC5 with a representative of each death-fold subfamily showed that NLRC5 fits best to the CARD subfamily and can be called an atypical CARD. Due to the specific structural features, the atypical CARD also displays a different electrostatic surface. Because the shape and charge of the surface is crucial for the establishment of a homotypic CARD-CARD interaction, these specific structural features seem to have a significant effect on the interaction between the atypical CARD of NLRC5 and the tandem RIG-I CARD.
PDB ID: 2MJMDownload
MMDB ID: 122792
PDB Deposition Date: 2014/1/12
Updated in MMDB: 2014/09
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2MJM: monomeric; determined by author
Molecular Components in 2MJM
Label Count Molecule
Protein (1 molecule)
Protein Nlrc5(Gene symbol: Nlrc5)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB