2MF6: Solution NMR structure of Chimeric Avidin, ChiAVD(I117Y), in the biotin bound form

Citation:
Abstract
Chimeric avidin (ChiAVD) is a product of rational protein engineering remarkably resistant to heat and harsh conditions. In quest of the fundamentals behind factors affecting stability we have elucidated the solution NMR spectroscopic structure of the biotin-bound form of ChiAVD and characterized the protein dynamics through 15N relaxation and hydrogen/deuterium (H/D) exchange of this and the biotin-free form. To surmount the challenges arising from the very large size of the protein for NMR spectroscopy, we took advantage of its high thermostability. Conventional triple resonance experiments for fully protonated proteins combined with methyl-detection optimized experiments acquired at 58 degrees C were adequate for the structure determination of this 56 kDa protein. The model-free parameters derived from the 15N relaxation data reveal a remarkably rigid protein at 58 degrees C in both the biotin-bound and the free forms. The H/D exchange experiments indicate a notable increase in hydrogen protection upon biotin binding.
PDB ID: 2MF6Download
MMDB ID: 122079
PDB Deposition Date: 2013/10/7
Updated in MMDB: 2014/08
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2MF6: tetrameric; determined by author
Molecular Components in 2MF6
Label Count Molecule
Proteins (4 molecules)
4
Avidin, Avidin-related Protein 4/5(Gene symbol: AVD)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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