2M98: NMR Structure of BeF3 Activated Sma0114

Bacterial receiver domains modulate intracellular responses to external stimuli in two-component systems. Sma0114 is the first structurally characterized representative from the family of receiver domains that are substrates for histidine-tryptophan-glutamate (HWE) kinases. We report the NMR structure of Sma0114 bound by Ca(2+) and BeF3(-), a phosphate analogue that stabilizes the activated state. Differences between the NMR structures of the inactive and activated states occur in helix alpha1, the active site loop that connects strand beta3 and helix alpha3, and in the segment from strand beta5 to helix alpha5 of the 455 (alpha4-beta5-alpha5) face. Structural rearrangements of the 455 face typically make receiver domains competent for binding downstream target molecules. In Sma0114 the structural changes accompanying activation result in a more negatively charged surface for the 455 face. Coupling between the 455 face and active site phosphorylation is usually mediated through the rearrangement of a threonine and tyrosine residue, in a mechanism called Y-T coupling. The NMR structure indicates that Sma0114 lacks Y-T coupling and that communication between the active site and the 455 face is achieved through a conserved lysine residue that stabilizes the acyl phosphate in receiver domains. (15)N-NMR relaxation experiments were used to investigate the backbone dynamics of the Sma0114 apoprotein, the binary Sma0114.Ca(2+) complex, and the ternary Sma0114.Ca(2+).BeF3(-) complex. The loss of entropy due to ligand binding at the active site is compensated by increased flexibility in the 455 face. The dynamic character of the 455 face in Sma0114, which results in part from the replacement of helix alpha4 by a flexible loop, may facilitate induced-fit recognition of target molecules.
PDB ID: 2M98Download
MMDB ID: 116936
PDB Deposition Date: 2013/6/4
Updated in MMDB: 2018/05
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2M98: monomeric; determined by author
Molecular Components in 2M98
Label Count Molecule
Protein (1 molecule)
Two-component Response Regulator
Molecule annotation
Chemicals (2 molecules)
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