2LQM: Solution Structures of RadA intein from Pyrococcus horikoshii

In protein splicing, an intervening protein sequence (intein) in the host protein excises itself out and ligates two split host protein sequences (exteins) to produce a mature host protein. Inteins require the involvement for the splicing of the first residue of the extein that follows the intein (which is Cys, Ser, or Thr). Other extein residues near the splicing junctions could modulate splicing efficiency even when they are not directly involved in catalysis. Mutual interdependence between this molecular parasite (intein) and its host protein (exteins) is not beneficial for intein spread but could be advantageous for intein survival during evolution. Elucidating extein-intein dependency has increasingly become important since inteins are recognized as useful biotechnological tools for protein ligation. We determined the structures of one of inteins with high splicing efficiency, the RadA intein from Pyrococcus horikoshii (PhoRadA). The solution NMR structure and the crystal structures elucidated the structural basis for its high efficiency and directed our efforts of engineering that led to rational design of a functional minimized RadA intein. The crystal structure of the minimized RadA intein also revealed the precise interactions between N-extein and the intein. We systematically analyzed the effects at the -1 position of N-extein and were able to significantly improve the splicing efficiency of a less robust splicing variant by eliminating the unfavorable extein-intein interactions observed in the structure. This work provides an example of how unveiling structure-function relationships of inteins offer a promising way of improving their properties as better tools for protein engineering.
PDB ID: 2LQMDownload
MMDB ID: 99614
PDB Deposition Date: 2012/3/9
Updated in MMDB: 2012/08
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2LQM: monomeric; determined by author
Molecular Components in 2LQM
Label Count Molecule
Protein (1 molecule)
PHO Rada Intein(Gene symbol: PH_RS01185)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB