2LON: Backbone structure of human membrane protein HIGD1B

Although nearly half of today's major pharmaceutical drugs target human integral membrane proteins (hIMPs), only 30 hIMP structures are currently available in the Protein Data Bank, largely owing to inefficiencies in protein production. Here we describe a strategy for the rapid structure determination of hIMPs, using solution NMR spectroscopy with systematically labeled proteins produced via cell-free expression. We report new backbone structures of six hIMPs, solved in only 18 months from 15 initial targets. Application of our protocols to an additional 135 hIMPs with molecular weight <30 kDa yielded 38 hIMPs suitable for structural characterization by solution NMR spectroscopy without additional optimization.
PDB ID: 2LONDownload
MMDB ID: 99788
PDB Deposition Date: 2012/1/26
Updated in MMDB: 2012/08
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2LON: monomeric; determined by author
Molecular Components in 2LON
Label Count Molecule
Protein (1 molecule)
Hig1 Domain Family Member 1B(Gene symbol: HIGD1B)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB