2LLJ: Structure Of A Bis-naphthalene Bound To A Thymine-thymine Dna Mismatch

The macrocyclic bis-naphthalene macrocycle (2,7-BisNP), belonging to the cyclobisintercalator family of DNA ligands, recognizes T-T mismatch sites in duplex DNA with high affinity and selectivity, as evidenced by thermal denaturation experiments and NMR titrations. The binding of this macrocycle to an 11-mer DNA oligonucleotide containing a T-T mismatch was studied using NMR spectroscopy and NMR-restrained molecular modeling. The ligand forms a single type of complex with the DNA, in which one of the naphthalene rings of the ligand occupies the place of one of the mismatched thymines, which is flipped out of the duplex. The second naphthalene unit of the ligand intercalates at the A-T base pair flanking the mismatch site, leading to encapsulation of its thymine residue via double stacking. The polyammonium linking chains of the macrocycle are located in the minor and the major grooves of the oligonucleotide and participate in the stabilization of the complex by formation of hydrogen bonds with the encapsulated thymine base and the mismatched thymine remaining inside the helix. The study highlights the uniqueness of this cyclobisintercalation binding mode and its importance for recognition of DNA lesion sites by small molecules.
PDB ID: 2LLJDownload
MMDB ID: 107670
PDB Deposition Date: 2011/11/10
Updated in MMDB: 2013/02
Experimental Method:
solution nmr
Source Organism:
Biological Unit for 2LLJ: dimeric; determined by author
Molecular Components in 2LLJ
Label Count Molecule
Nucleotides(2 molecules)
DNA (5'-d(*cp*gp*tp*cp*gp*tp*ap*gp*tp*gp*c)-3')
Molecule annotation
DNA (5'-d(*gp*cp*ap*cp*tp*tp*cp*gp*ap*cp*g)-3')
Molecule annotation
Chemical (1 molecule)
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