2L2N: Backbone 1H, 13C, and 15N Chemical Shift Assignments for the first dsRBD of protein HYL1

HYL1 is a double-stranded RNA binding protein involved in microRNA processing in plants. HYL1 enhances the efficiency and precision of the RNase III protein DCL1 and participates in microRNA strand selection. In this work, we dissect the contributions of the domains of HYL1 to the binding of RNA targets. We found that the first domain is the main contributor to RNA binding. Mapping of the interaction regions by nuclear magnetic resonance on the structure of HYL1 RNA-binding domains showed that the difference in binding capabilities can be traced to sequence divergence in beta2-beta3 loop. The possible role of each domain is discussed in light of previous experimental data.
PDB ID: 2L2NDownload
MMDB ID: 85114
PDB Deposition Date: 2010/8/23
Updated in MMDB: 2010/10
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2L2N: monomeric; determined by author
Molecular Components in 2L2N
Label Count Molecule
Protein (1 molecule)
Hyponastic Leave 1(Gene symbol: HYL1)
Molecule annotation
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Citing MMDB