2KWI: RalB-RLIP76 (RalBP1) complex

RLIP76 (RalBP1) is a multidomain protein that interacts with multiple small G protein families: Ral via a specific binding domain, and Rho and R-Ras via a GTPase activating domain. RLIP76 interacts with endocytosis proteins and has also been shown to behave as a membrane ATPase that transports chemotherapeutic agents from the cell. We have determined the structure of the Ral-binding domain of RLIP76 and show that it comprises a coiled-coil motif. The structure of the RLIP76-RalB complex reveals a novel mode of binding compared to the structures of RalA complexed with the exocyst components Sec5 and Exo84. RLIP76 interacts with both nucleotide-sensitive regions of RalB, and key residues in the interface have been identified using affinity measurements of RalB mutants. Sec5, Exo84, and RLIP76 bind Ral proteins competitively and with similar affinities in vitro.
PDB ID: 2KWIDownload
MMDB ID: 84482
PDB Deposition Date: 2010/4/12
Updated in MMDB: 2010/09
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 2KWI: dimeric; determined by author
Molecular Components in 2KWI
Label Count Molecule
Proteins (2 molecules)
Ras-related Protein Ral-b(Gene symbol: RALB)
Molecule annotation
Rala-binding Protein 1(Gene symbol: RALBP1)
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB