2KOG: lipid-bound synaptobrevin solution NMR structure

The synaptic vesicle protein synaptobrevin engages with syntaxin and SNAP-25 to form the SNARE complex, which drives membrane fusion in neuronal exocytosis. In the SNARE complex, the SNARE motif of synaptobrevin forms a 55-residue helix, but it has been assumed to be mostly unstructured in its prefusion form. NMR data for full-length synaptobrevin in dodecylphosphocholine micelles reveals two transient helical segments flanked by natively disordered regions and a third more stable helix. Transient helix I comprises the most N-terminal part of the SNARE motif, transient helix II extends the SNARE motif into the juxtamembrane region, and the more stable helix III is the transmembrane domain. These helices may have important consequences for SNARE complex folding and fusion: helix I likely forms a nucleation site, the C-terminal disordered SNARE motif may act as a folding arrest signal, and helix II likely couples SNARE complex folding and fusion.
PDB ID: 2KOGDownload
MMDB ID: 78388
PDB Deposition Date: 2009/9/22
Updated in MMDB: 2009/12
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 2KOG
Label Count Molecule
Protein (1 molecule)
Vesicle-associated Membrane Protein 2(Gene symbol: Vamp2)
Molecule annotation
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Citing MMDB