2KFK: Solution structure of Bem1p PB1 domain complexed with Cdc24p PB1 domain

Bem1 and Cdc24 of the budding yeast Saccharomyces cerevisiae interact with each other through PB1-PB1 heterodimer formation to regulate the establishment of cell polarity. Here we present the tertiary structure of the heterodimer of Bem1 and Cdc24 PB1 domains determined by NMR spectroscopy. To avoid ambiguity in the NMR spectral analysis, we first prepared a mutant of the Cdc24 PB1 domain that had truncated loops. The mutant provided well dispersed spectra without spectral overlapping, thus allowing unambiguous spectral assignments for structure determination. We confirmed that the loop deletion-mutant was quite similar to the wild-type in both 3D structure and binding affinity. The NMR structure of the heterodimer of the deletion-mutant of Cdc24 PB1 and Bem1 PB1 was determined using a variety of isotope labelled samples including perdeuteration. The interface between the Bem1/Cdc24 PB1 heterodimer was analysed at atomic resolution. Through a comparison with the tertiary structures of other PB1-PB1 heterodimers, we found that conserved electrostatic properties on the molecular surface were commonly used for PB1-PB1 interaction, but hydrophobic interactions were important for cognate interaction in Bem1/Cdc24 PB1 heterodimer formation.
PDB ID: 2KFKDownload
MMDB ID: 77102
PDB Deposition Date: 2009/2/23
Updated in MMDB: 2009/10
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 2KFK
Label Count Molecule
Proteins (2 molecules)
BUD Emergence Protein 1(Gene symbol: BEM1)
Molecule annotation
Cell Division Control Protein 24(Gene symbol: CDC24)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB