2KEQ: Solution structure of DnaE intein from Nostoc punctiforme

Naturally split DnaE intein from Nostoc punctiforme (Npu) has robust protein trans-splicing activity and high tolerance of sequence variations at the splicing junctions. We determined the solution structure of a single chain variant of NpuDnaE intein by NMR spectroscopy. Based on the NMR structure and the backbone dynamics of the single chain NpuDnaE intein, we designed a functional split variant of the NpuDnaE intein having a short C-terminal half (C-intein) composed of six residues. In vivo and in vitro protein ligation of model proteins by the newly designed split intein were demonstrated.
PDB ID: 2KEQDownload
MMDB ID: 72687
PDB Deposition Date: 2009/2/2
Updated in MMDB: 2018/05
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Molecular Components in 2KEQ
Label Count Molecule
Protein (1 molecule)
DNA Polymerase III Alpha Subunit, Nucleic Acid Binding Ob-fold Trna/helicase-type
Molecule annotation
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