2K9F: Structural features of the complex between the DsbD N-terminal and the PilB N-terminal domains from Neisseria meningitidis

Citation:
Abstract
DsbD transmembrane protein dispatches electrons to periplasmic Trx/DsbE-like partners via specific interactions with its N-terminal domain, nDsbD. In the present study, PilB N-terminal domain (NterPilB) is shown to efficiently accept electrons coming from nDsbD from Neisseria meningitidis. Using an NMR-driven docking approach, we have modeled the structure of a mixed disulfide complex between NterPilB and nDsbD. We show the needed opening of nDsbD cap-loop whereas NterPilB FLHE loop does not seem essential in the formation and stabilization of the complex. Relaxation analysis performed on backbone amide groups highlights a kind of dynamics transfer from nDsbD cap-loop on NterPilB alpha1 helix, suggesting that a mobility contribution is required not only for the formation of the mixed disulfide complex, but also for its disruption. Taking into account previous X-ray data on covalent complexes involving nDsbD, a cartoon of interactions between Trx-like partners and nDsbD is proposed that illustrates the adaptability of nDsbD.
PDB ID: 2K9FDownload
MMDB ID: 72683
PDB Deposition Date: 2008/10/9
Updated in MMDB: 2009/09
Experimental Method:
solution nmr
Source Organism:
Neisseria meningitidis serogroup A
Similar Structures:
Molecular Components in 2K9F
Label Count Molecule
Proteins (2 molecules)
1
Thioredoxin
Molecule annotation
1
Thiol:disulfide Interchange Protein Dsbd(Gene symbol: dipZ)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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