2K0T: High Resolution Solution Nmr Structures Of Oxaliplatin-Dna Adduct

The differences in efficacy and molecular mechanisms of platinum anti-cancer drugs cisplatin (CP) and oxaliplatin (OX) are thought to be partially due to the differences in the DNA conformations of the CP and OX adducts that form on adjacent guanines on DNA, which in turn influence the binding of damage-recognition proteins that control downstream effects of the adducts. Here we report a comprehensive comparison of the structural distortion of DNA caused by CP and OX adducts in the TGGT sequence context using nuclear magnetic resonance (NMR) spectroscopy and molecular dynamics (MD) simulations. When compared to our previous studies in other sequence contexts, these structural studies help us understand the effect of the sequence context on the conformation of Pt-GG DNA adducts. We find that both the sequence context and the type of Pt-GG DNA adduct (CP vs. OX) play an important role in the conformation and the conformational dynamics of Pt-DNA adducts, possibly explaining their influence on the ability of many damage-recognition proteins to bind to Pt-DNA adducts.
PDB ID: 2K0TDownload
MMDB ID: 69360
PDB Deposition Date: 2008/2/14
Updated in MMDB: 2011/09
Experimental Method:
solution nmr
Source Organism:
Biological Unit for 2K0T: dimeric; determined by author
Molecular Components in 2K0T
Label Count Molecule
Nucleotides(2 molecules)
DNA (5'-d(*dcp*dcp*dtp*dcp*dtp*dgp*dgp*dtp*dcp*dtp*dcp*dc)- 3')
Molecule annotation
DNA (5'-d(*dgp*dgp*dap*dgp*dap*dcp*dcp*dap*dgp*dap*dgp*dg)- 3')
Molecule annotation
Chemical (1 molecule)
* Click molecule labels to explore molecular sequence information.

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