2JPP: Structural basis of RsmA/CsrA RNA recognition: Structure of RsmE bound to the Shine-Dalgarno sequence of hcnA mRNA

Proteins of the RsmA/CsrA family are global translational regulators in many bacterial species. We have determined the solution structure of a complex formed between the RsmE protein, a member of this family from Pseudomonas fluorescens, and a target RNA encompassing the ribosome-binding site of the hcnA gene. The RsmE homodimer with its two RNA-binding sites makes optimal contact with an 5'-A/UCANGGANGU/A-3' sequence in the mRNA. When tightly gripped by RsmE, the ANGGAN core folds into a loop, favoring the formation of a 3-base-pair stem by flanking nucleotides. We validated these findings by in vivo and in vitro mutational analyses. The structure of the complex explains well how, by sequestering the Shine-Dalgarno sequence, the RsmA/CsrA proteins repress translation.
PDB ID: 2JPPDownload
MMDB ID: 59088
PDB Deposition Date: 2007/5/21
Updated in MMDB: 2007/10
Experimental Method:
solution nmr
Source Organism:
Pseudomonas fluorescens
Similar Structures:
Molecular Components in 2JPP
Label Count Molecule
Proteins (2 molecules)
Translational Repressor
Molecule annotation
Nucleotide(1 molecule)
RNA (5'-r(*gp*gp*gp*cp*up*up*cp*ap*cp*gp*gp*ap*up*gp*ap*ap*gp*cp*cp*c)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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